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The main symptoms include: Extreme tiredness Lack of energy Red or sore tongue or mouth Pale skin Changes in mood Changes in movement and balance Feeling pins and needles in arms and feet Reasons for vitamin b12 deficiency Vitamin b12 deficiency is caused mainly by two reasons

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At these time points, alamarBlue (Sigma-Aldrich) was added at 0.4% concentration and incubated for 2 h (37 C and 5% CO 2 )

Animals which underwent the manoeuvre of ischemic preconditioning showed significantly lower degrees of changes in the levels of neurotransmitter/ (amino acids), supporting the induction of the proposed protective tolerance effect

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